桂花色泽关键基因OfCCD4互作转录因子的鉴定分析OA北大核心CSTPCD

[Objective]Defining the transcription factors of OfCCD4,a key gene that regulates carotenoid metabolism in Osmanthus fragrans,as well as exploring the molecular mechanism of transcription factors binding to OfCCD4 and regulating OfCCD4 expression,which provide a theoretical basis for revealing the molecular mechanism of carotenoids metabolism as well as for O.fragrans color genetic engineering breeding.[Method]The petals of Osmanthus flower were obtained in Taihuyuan,which located in Lin'an,Hangzhou City.The transcription factors of OfCCD4 were initially predicted through the online websites,and further identified by real-time fluorescence quantitative(RT-qPCR)and yeast-one-hybrid assays.[Result]Transcriptome analysis were that 10 transcription factors were highly correlated with the expression level of OfCCD4.Transactivation activity in Y1H yeast showed that 8 transcription factors of them had transactivation activity.Finally,3 transcription factors displayed strong interaction and 3 transcription factors exhibited weak interaction with OfCCD4 promoter in yeast-one-hybrid assay.[Conclusion]In this study,three transcription factors with transactivation activity and strong interaction with OfCCD4 were screened,indicating they might affect flower color by regulating OfCCD4 expression in Osmanthus fragrans.