河南农业大学学报2024，Vol.58Issue(5)：757-764,8.DOI:10.16445/j.cnki.1000-2340.20240418.001
桂花色泽关键基因OfCCD4互作转录因子的鉴定分析
Identification of transcriptome-based Osmanthus fragrans OfCCD4 interaction transcription factors
摘要
Abstract
[Objective]Defining the transcription factors of OfCCD4,a key gene that regulates carotenoid metabolism in Osmanthus fragrans,as well as exploring the molecular mechanism of transcription factors binding to OfCCD4 and regulating OfCCD4 expression,which provide a theoretical basis for revealing the molecular mechanism of carotenoids metabolism as well as for O.fragrans color genetic engineering breeding.[Method]The petals of Osmanthus flower were obtained in Taihuyuan,which located in Lin'an,Hangzhou City.The transcription factors of OfCCD4 were initially predicted through the online websites,and further identified by real-time fluorescence quantitative(RT-qPCR)and yeast-one-hybrid assays.[Result]Transcriptome analysis were that 10 transcription factors were highly correlated with the expression level of OfCCD4.Transactivation activity in Y1H yeast showed that 8 transcription factors of them had transactivation activity.Finally,3 transcription factors displayed strong interaction and 3 transcription factors exhibited weak interaction with OfCCD4 promoter in yeast-one-hybrid assay.[Conclusion]In this study,three transcription factors with transactivation activity and strong interaction with OfCCD4 were screened,indicating they might affect flower color by regulating OfCCD4 expression in Osmanthus fragrans.关键词
桂花/类胡萝卜素/OfCCD4基因/转录因子/酵母单杂交Key words
Osmanthus fragrans/carotenoid/OfCCD4 gene/transcription factors/yeast-one-hybrid分类
农业科技引用本文复制引用
史德静,邬俊媛,韩柳青,张瑞珍,林楠,尚富德,刘燕培..桂花色泽关键基因OfCCD4互作转录因子的鉴定分析[J].河南农业大学学报,2024,58(5):757-764,8.基金项目
河南省重大公益专项项目(201300110900) （201300110900）
全国重点实验室开放课题资助项目(Horti-KF-2023-10) （Horti-KF-2023-10）
河南省青年科学基金项目(222300420183) （222300420183）
河南农业大学科技创新基金项目(KJCX2021A17) （KJCX2021A17）
河南省留学人员科研择优资助项目(30602425) （30602425）
