普洱茶渥堆发酵中可降解咖啡碱真菌菌株的筛选和鉴定OA北大核心CSCDCSTPCD
Screen and Identification of Fungi Strain Degrading Caffeine in Pu-erh Tea during Solid-state Fermentation
为了在普洱茶渥堆发酵中筛选出可降解咖啡碱的菌株,采用咖啡碱液体筛选培养基从不同阶段普洱茶发酵样中筛选目标菌株,结合菌落特征、分生孢子结构以及18 S rDNA序列对目标菌株进行鉴定.并将目标菌株接种至茶叶内进行单菌种发酵,通过高效液相色谱法(HPLC)测定咖啡碱、茶碱、可可碱、3-甲基黄嘌呤等嘌呤碱含量.结果表明,在普洱茶发酵样中筛选出一株有效降解咖啡碱的菌株,此菌株在48 h内对咖啡碱的降解率为87.7%.经鉴定为聚多曲霉(Aspergillu…查看全部>>
To identify fungi strain involved in caffeine degradation in Pu-erh tea, caffeine liquid medium was used to screen target strain in Pu-erh tea of different stages during solid-state fermentation. The candidate strains were then analyzed for colony morphology, morphological characteristics and 18 S rDNA. The target strain was inoculated into tea for fermentation. Caffeine, theophylline, theobromine and 3-methylxanthine were determined by HPLC during the ferme…查看全部>>
马存强;周斌星;王洪振;王潘
云南农业大学龙润普洱茶学院,云南 昆明 650201昆明大朴茶业有限公司,云南 昆明 650224云南农业大学龙润普洱茶学院,云南 昆明 650201安徽农业大学茶与食品科技学院,安徽 合肥 230036
轻工纺织
聚多曲霉咖啡碱茶碱普洱茶渥堆发酵
Aspergillus sydowiicaffeinetheophyllinePu-erh teasolid-state fermentation
《茶叶科学》 2017 (2)
211-219,9
紫鹃茶树调控花色苷生物合成的 MBW 转录因子复合体研究(C161104)、中国现代农业产业技术体系(CARS-23)
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