中国食品学报2023，Vol.23Issue(11)：46-55,10.DOI:10.16429/j.1009-7848.2023.11.005
鸭梨PbADC基因的鉴定及在果心褐变中的表达
Identification and Expression Analysis of PbADC Gene in Core Browning of Yali Pear
摘要
Abstract
The purpose of this study was to explore the regulatory role of arginine decarboxylase(ADC)in core browning of Yali pear.Using Yali pear as material,the arginine decarboxylase of Pyrus bretschneideri(PbADC)was identified,and the bioinformation of PbADC and the gene expression pattern of PbADC were analyzed.The results showed that the PbADC gene was successfully identified and contained a complete open reading frame of 2 193 bp that encoding 730 amino acids.The phylogenetic analysis showed that PbADC was very closely to ADC of Pyrus betulifolia,Malus domesti-ca,Pyrus ussuriensis × Pyrus communis and Malus hup e he ns is,and the multi alignment analysis showed that PbADC containing the complete Orn_Arg_deC_N domain,the binding site of pyridoxal 2-phosphate of ADC family and two im-portant enzyme activity sites.Bioinformatics analysis showed that PbADC was a hydrophilic unstable protein with theoreti-cal isoelectric point of 5.23 and molecular weight of 78.403 ku,which was hydrophilic and non-transmembrane without signal peptide,and α-helix and random coil were the main secondary structure of PbADC.The core browning index of rapid cooling treatment group was higher than that of slow cooling treatment group.Quantitative PCR and enzyme activity results showed that the expression of PbADC increased at the early storange period and then decreased during the brown-ing of Yali pear,and the change of ADC enzyme activity was consistent with the gene expression level.This study clari-fied the bioinformatics characteristics of PbADC protein and the differential expression of PbADC gene during storage,which provided a theoretical basis for further research on the mechanism of postharvest browning of Yali pear.关键词
鸭梨/褐变/精氨酸脱羧酶基因/基因鉴定/表达分析Key words
Yali pear/browning/arginine decarboxylase gene/gene identification/expression analysis引用本文复制引用
李庆秀,邓冰,寇晓敏,李萍,吴海清,梁丽雅,闫师杰..鸭梨PbADC基因的鉴定及在果心褐变中的表达[J].中国食品学报,2023,23(11):46-55,10.基金项目
国家自然科学基金面上项目(32072278) （32072278）
天津市自然科学基金重点项目(20JCZDJC00420) （20JCZDJC00420）
