食品科学2024，Vol.45Issue(18)：1-9,9.DOI:10.7506/spkx1002-6630-20240105-057
NO介导的宰后成熟期间滩羊肉线粒体通路细胞凋亡对嫩度的影响
Effect of Nitric Oxide(NO)-Mediated Mitochondrial Apoptosis on the Tenderness of Tan Sheep Meat during Postmortem Aging
摘要
Abstract
The aim of this study was to assess the effect of nitric oxide(NO)-mediated mitochondrial apoptosis on Tan sheep meat tenderness during postmortem aging.The hind leg muscle of Tan sheep was injected with 0.8%NaCl(control),20 mmol/L aminoguanidine hydrochloride(AH)or 90 mmol/L L-arginine(L-Arg)and aged at 4℃for 0,6,12,24,48,96,and 192 h.NO and inducible nitric oxide synthase(iNOS)contents,cytochrome c(Cyt c)redox state,oxidative stress level,mitochondrial apoptotic pathways,and tenderness were measured during postmortem aging.The results indicated that at 12 and 24 h after slaughter,NO and iNOS levels were significantly higher,and ATP levels were significantly lower in the L-Arg group than in the control group(P<0.05).At 24 and 48 h,Cyt c reduction levels and superoxide dismutase(SOD)activity were significantly higher,while the level of reactive oxygen species(ROS)was significantly lower in the AH group than in the control group(P<0.05).From 12 to 96 h,ATP contents were significantly lower in the L-Arg group than in the control group(P<0.05).In addition,at 24 h,Ca2+concentration significantly increased in the L-Arg group compared with the control group(P<0.05).At all time points except 24 h,the caspase-3 activity in the L-Arg group was significantly higher than that in the control group(P<0.05).Moreover,it was found that the Bax/Bcl-2 ratio,pH,and myofibril fragmentation index(MFI)in the L-Arg group were significantly higher than those in the control group at all time points after slaughter(P<0.05).Shear force was significantly lower in the L-Arg group but significantly higher in the AH group than in the control group(P<0.05).In conclusion,NO exacerbates mitochondrial oxidative stress and directly upregulates ROS levels during the postmortem aging of Tan sheep meat,leading to a decrease in SOD activity,irreversible opening of mitochondrial permeability transition pore(MPTP),and ultimately mitochondrial damage.In addition,by controlling the redox state of Cyt c,NO indirectly activates caspase-3 activity to accelerate apoptotic energy metabolism,deplete ATP and consequently maintain myocyte homeostasis.Furthermore,NO affects the release of Ca2+,up-regulates the Bax/Bcl-2 ratio,and induces the occurrence of mitochondrial apoptotic cascade,ultimately resulting in an increase in MFI and a decrease in shear force and,consequently,improving the tenderness of Tan sheep meat.关键词
嫩度/NO/滩羊肉/细胞凋亡Key words
tenderness/nitric oxide/Tan sheep meat/cell apoptosis分类
轻工业引用本文复制引用
马佳荣,高爽,刘长玲,梁艳群,虎瑶,罗瑞明..NO介导的宰后成熟期间滩羊肉线粒体通路细胞凋亡对嫩度的影响[J].食品科学,2024,45(18):1-9,9.基金项目
国家自然科学基金地区科学基金项目(32260555) （32260555）
