中国食品学报2023，Vol.23Issue(11)：212-222,11.DOI:10.16429/j.1009-7848.2023.11.021
低温等离子体对气调包装中荧光假单胞菌的抑杀机理
Inhibition Mechanism of Cold Atmospheric Plasma on Pseudomonas fluorescens in Modified Atmosphere Packaging
摘要
Abstract
In order to investigate the bactericidal conditions of cold atmospheric plasma(CAP)on Pseudomonas fluorescen in modified atmosphere packaging(MAP)and the bactericidal mechanism of CAP in MAP,three factor and three levels orthogonal test was used to optimize the conditions of CAP treatment.The inhibitory mechanism of CAP against Pseu-domonas fluorescens was studied by cell activity,cell wall permeability,cell membrane integrity,antioxidant enzyme ac-tivity and microstructure.The results showed that the best sterilization conditions were as follows:Treatment voltage as 50 kV,treatment time as 180s,modified atmosphere volume ratio as V(N2)∶V(CO2)=3∶2,under these conditions,the steril-ization rate was 99.49％.The inhibitory mechanism of CAP on Pseudomonas fluorescens showed that cold atmospheric plasma treatment reduced cell activity.The integrity of cell wall and cell membrane was damaged,and the leakage of nucleic acid and protein increased,Na+/K+-ATPase(ATP)content decreased by 13.10 U/mg prot,Propidium iodide(PI)increased,and alkaline phosphatase(AKP)activity decreased to 12.09 King unit/g prot.The activities of antioxidant en-zymes superoxide dismutase(SOD)and catalase(CAT)in cells decreased.It changes the shape of the cells,making them stick and sag.In conclusion,cold atmospheric plasma combined with modified atmosphere packaging could effectively in-hibit Pseudomonas fluorescens,resulting in cell inactivation or death,and the longer the treatment time,the better the effect.关键词
低温等离子体/荧光假单胞菌/抑菌机理/气调包装Key words
cold atmospheric plasma/Pseudomonas fluorescens/antibacterial mechanism/modified atmosphere packaging引用本文复制引用
徐慧倩,胡家杰,邓尚贵,缪文华..低温等离子体对气调包装中荧光假单胞菌的抑杀机理[J].中国食品学报,2023,23(11):212-222,11.基金项目
国家重点研发计划项目(2021YFD2100500) （2021YFD2100500）
省属高校基本科研业务费青年创新专项/重点项目(2021JZ007) （2021JZ007）
