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低温等离子体对气调包装中荧光假单胞菌的抑杀机理OACSCD

Inhibition Mechanism of Cold Atmospheric Plasma on Pseudomonas fluorescens in Modified Atmosphere Packaging

中文摘要英文摘要

为探讨低温等离子体(CAP)对气调包装中荧光假单胞菌的杀菌条件以及CAP在气调包装中的杀菌机理,以杀菌率为指标,采用三因素三水平正交试验法优化CAP处理条件;采用细胞活性、细胞壁通透性、细胞膜完整性、抗氧化酶活性及微观结构等指标研究CAP对荧光假单胞菌的抑菌机理.结果显示:CAP协同气调包装的最佳杀菌条件为处理电压50 kV、处理时间180s、气调气体体积比V(N2)∶V(C02)=3∶2,在此条件下杀菌率为99.49%.CAP对荧光假单胞菌的抑菌机理表明,低温等离子体处理使细胞活性降低;破坏了细胞壁和细胞膜的完整性,核酸和蛋白质泄露量增加,Na+/K+-ATP酶(ATP)下降了 13.10U/mg prot,碘化丙啶(PI)升高,碱性磷酸酶(AKP)活力下降为12.09金氏单位/g prot;细胞内抗氧化酶超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性下降;改变了细胞的形态,使细胞黏连和凹陷.结论:低温等离子体协同气调包装可有效抑杀荧光假单胞菌,致使细胞失活或死亡,且处理时间越长效果越好.

In order to investigate the bactericidal conditions of cold atmospheric plasma(CAP)on Pseudomonas fluorescen in modified atmosphere packaging(MAP)and the bactericidal mechanism of CAP in MAP,three factor and three levels orthogonal test was used to optimize the conditions of CAP treatment.The inhibitory mechanism of CAP against Pseu-domonas fluorescens was studied by cell activity,cell wall permeability,cell membrane integrity,antioxidant enzyme ac-tivity and microstructure.The results showed that the best sterilization conditions were as follows:Treatment voltage as 50 kV,treatment time as 180s,modified atmosphere volume ratio as V(N2)∶V(CO2)=3∶2,under these conditions,the steril-ization rate was 99.49%.The inhibitory mechanism of CAP on Pseudomonas fluorescens showed that cold atmospheric plasma treatment reduced cell activity.The integrity of cell wall and cell membrane was damaged,and the leakage of nucleic acid and protein increased,Na+/K+-ATPase(ATP)content decreased by 13.10 U/mg prot,Propidium iodide(PI)increased,and alkaline phosphatase(AKP)activity decreased to 12.09 King unit/g prot.The activities of antioxidant en-zymes superoxide dismutase(SOD)and catalase(CAT)in cells decreased.It changes the shape of the cells,making them stick and sag.In conclusion,cold atmospheric plasma combined with modified atmosphere packaging could effectively in-hibit Pseudomonas fluorescens,resulting in cell inactivation or death,and the longer the treatment time,the better the effect.

徐慧倩;胡家杰;邓尚贵;缪文华

浙江海洋大学食品与药学学院 浙江舟山 316022

低温等离子体荧光假单胞菌抑菌机理气调包装

cold atmospheric plasmaPseudomonas fluorescensantibacterial mechanismmodified atmosphere packaging

《中国食品学报》 2023 (011)

212-222 / 11

国家重点研发计划项目(2021YFD2100500);省属高校基本科研业务费青年创新专项/重点项目(2021JZ007)

10.16429/j.1009-7848.2023.11.021

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