中国食品学报2023，Vol.23Issue(12)：143-152,10.DOI:10.16429/j.1009-7848.2023.12.016
基于定量构效关系制备火麻仁蛋白ACE抑制肽
Preparation of ACE Inhibitory Peptide of Hemp Seed Protein Based on Quantitative Structure-Activity Relationship
摘要
Abstract
High blood pressure is a major risk factor for cardiovascular disease,and frequent use of blood pressure med-ications can have side effects.Therefore,safe and efficient food-borne antihypertensive peptides have become the focus of attention.In this study,a quantitative structure-activity relationship(QSAR)model of angiotensin-converting enzyme(ACE)inhibitory peptide was established to determine the relationship between the structure and activity of ACE in-hibitory peptide.The model results were combined with computer virtual enzyme digestion to determine proteolytic sites accurately.Suitable proteases were selected to hydrolyze hemp seed protein to prepare ACE inhibitory peptide,and the inhibitory effect of the final hydrolysate was determined.The results showed that the SVM-AAindex model based on dipeptide had the best predictive performance(R2 0.81,RMSE 0.53),and had a strong ACE inhibition effect when the C-terminal amino acids of dipeptide were hydrophobic and aromatic amino acids.The analysis of protein components showed that hemp seed protein contains 34.60％hydrophobic amino acids and aromatic amino acids,which is a good source for preparing ACE inhibitory peptides.The alkaline and subtilisin,which produced more short peptides and bioac-tive peptides by virtual enzyme digestion,were selected for directed hydrolysis of hemp seed protein.The ACE inhibition values of the final hydrolysates were 1.89 and 2.30 mg/mL,respectively.In this study,an efficient and accurate method for preparing ACE inhibitory peptide from hemp seed protein was established,which has a good application prospect in antihypertensive drugs.关键词
火麻仁蛋白/ACE抑制肽/QSAR模型/定向制备Key words
hemp seed protein/ACE inhibitory peptide/QSAR model/directed preparation引用本文复制引用
刘梦琪,刘畅,刘博浩,阴雨奇,张瀚林,张英华,董艳..基于定量构效关系制备火麻仁蛋白ACE抑制肽[J].中国食品学报,2023,23(12):143-152,10.基金项目
黑龙江省现代农业产业技术协同创新推广体系麻类(工业)(SCXTI(MLGY)2019) （工业）
