小白杏生理落果中多酚提取及体外抗氧化活性分析OA北大核心CSTPCD

[Objective]In this study,the extraction process of polyphenols from Armeniaca vulgaris Lam.during physiological fruit-falling period was optimized and the antioxidant activity of the polyphenol ex-tract was analyzed.[Methods]On the basis of single factor experiments,response surface method was used to determine the optimum process.The antioxidant activity in vitro was assayed with spectrophotometric method.[Results]the suitable extraction conditions were ethanol volume fraction 70%,solid-liquid ratio 1∶21,ex-traction time 34 minute and temperature 60℃.Under these conditions,the polyphenol extraction yield was 12.50 mg/g.The relative standard deviation of the proved experiment was 1.31%compared with the predicted value,which indicated the regression model was suitable for the polyphenol extraction from A.vulgaris.during physiological fruit-falling period.The IC50 of scavenging the free radicals of ABTS+,DPPH,O2-were 63.55,55.84,103.00 μg/mL respectively.The results indicated that the ability of the polyphenol extraction to scav-enge free radicals in the order was DPPH>ABTS+>O2-.There is slightly lower scavenging ability of NO2-.[Conclusion]The findings would lays the foundation for the development and utilization of A.vulgaris.during physiological fruit-falling period in anti-free radical oxidation functional food.